污水处理厂接种物的多样性及对化学品生物降解的影响

doi:10.11934/j.issn.1673-4831.2016.01.017

生态与农村环境学报 ›› 2016, Vol. 32 ›› Issue (1): 102-109.doi: 10.11934/j.issn.1673-4831.2016.01.017

污水处理厂接种物的多样性及对化学品生物降解的影响

林春骏^1,2, 周林军², 刘济宁², 杨倩², 石利利^1,2

1. 南京信息工程大学环境科学与工程学院, 江苏南京 210044;
2. 环境保护部南京环境科学研究所, 江苏南京 210042

收稿日期:2015-03-25 出版日期:2016-01-25 发布日期:2016-01-27
通讯作者: 石利利,通信作者E-mail:sll@nies.org E-mail:sll@nies.org
作者简介:林春骏(1990-),男,江苏南京人,硕士生,主要从事化学品生态效应测试方面的研究。E-mail:lincj@aliyun.com
基金资助:
国家高技术研究发展计划(2013AA060A308);环保公益性行业科研专项(2013467028);中央级公益性科研院所基本科研业务专项

Diversification of Inocula and Its Effect on Biodegradation of Chemicals in Sewage Treatment Plant

LIN Chun-jun^1,2, ZHOU Lin-jun², LIU Ji-ning², YANG Qian², SHI Li-li^1,2

1. School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, China;
2. Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China

Received:2015-03-25 Online:2016-01-25 Published:2016-01-27

摘要/Abstract

摘要：

生物降解是化学品在环境中的主要去除途径,接种物来源和特性是快速生物降解性测试结果产生差异的重要因素。从广州和贵阳市6家生活污水处理厂采集活性污泥和二级出水,分别培养得到64和52株细菌,共有菌属为气单胞菌(Aeromonas)、芽孢杆菌(Bacillus)和肠杆菌(Enterobacter)。变性梯度凝胶电泳结果显示,广州市3家污水处理厂活性污泥和二级出水条带数量分别为53~58和38~43条;而贵阳市3家污水处理厂分别为29~42和30~42条。6种来源的接种物对易降解的萘甲酸和难降解的4-硝基苄氯生物降解测试结果较一致,接种物来源差异对中等降解性的联苯生物降解结果影响显著,其接种物多样性与生物降解性呈正相关关系。

关键词: 活性污泥, 接种物, 化学品, 生物降解, 生物多样性

Abstract:

Biodegradation is a major means to eliminate chemicals in environment. Sources and bio-characteristics of inocula are the major factors affecting biodegradability of chemicals. Activated sludge and secondary effluent was collected from 6 sewage treatment plants in Guangzhou and Guiyang City and cultivated for isolation of biodegrading bacteria. A total of 64 and 52 strains of bacteria were isolated from the sludge and effluent collected in Guangzhou and Guiyang City, respectively, Aeromonas, Bacillus, and Enterobacter were common strains included in the two groups. Polymerase chain reaction analysis shows that 53-58 and 38-43 bands were detected in the sludge and effluent, respectively, collected from the three sewage treatment plants in Guangzhou City, and 29-42 and 30-42 bands in the sludge and effluent, respectively, collected from the three sewage treatment plants in Guiyang City. They were used as inocula for biodegradation of chemicals. Test of the inocula from 6 different sources biodegrading readily degradable naphthoic acid and hardly degradable 4-nitro benzyl chloride show that they were more or less the same in degrading effect, but they varied in effect of degrading moderately degradable biphenyl and 2,4-dichlorophenol,which displayed inhibitory effects on the inocula. All the findings show that biodegradation of the pollutants is positively related to diversity of the inocula.

Key words: activated sludge, inoculum, chemicals, biodegradation, biodiversity

中图分类号:

X703

林春骏, 周林军, 刘济宁, 杨倩, 石利利. 污水处理厂接种物的多样性及对化学品生物降解的影响[J]. 生态与农村环境学报, 2016, 32(1): 102-109.

LIN Chun-jun, ZHOU Lin-jun, LIU Ji-ning, YANG Qian, SHI Li-li. Diversification of Inocula and Its Effect on Biodegradation of Chemicals in Sewage Treatment Plant[J]. Journal of Ecology and Rural Environment, 2016, 32(1): 102-109.

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污水处理厂接种物的多样性及对化学品生物降解的影响

Diversification of Inocula and Its Effect on Biodegradation of Chemicals in Sewage Treatment Plant

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