生态与农村环境学报 ›› 2015, Vol. 31 ›› Issue (4): 528-533.doi: 10.11934/j.issn.1673-4831.2015.04.013

• 自然保护与生态 • 上一篇    下一篇

贫瘠营养条件下转cry1Ab/c基因水稻的生态适合度

付健美,方志翔,郭汝清,刘标   

  1. 南京农业大学生命科学学院
  • 收稿日期:2015-01-26 修回日期:2015-04-16 出版日期:2015-07-25 发布日期:2015-07-25
  • 通讯作者: 刘标 E-mail:liubiao@nies.org;
  • 作者简介:付健美(1990-),女,山西吕梁人,硕士生,主要从事转基因生物安全风险评估方面的研究。E-mail:243420660@qq.com
  • 基金资助:

    国家自然科学基金(31370544); 转基因新品种培育重大科技专项(2014ZX08012-005); 环保公益性行业科研专项(201509044)

Ecological Fitness of Transgenic cry1Ab/c Rice in Nutrient-Deficient Soils

FU Jian-mei,FANG Zhi-xiang,GUO Ru-qing,LIU Biao   

  1. College of Life Sciences, Nanjing Agricultural University
  • Received:2015-01-26 Revised:2015-04-16 Online:2015-07-25 Published:2015-07-25

摘要:

以抗虫转cry1 Ab/c基因水稻华恢1号(Bt水稻)及其非转基因亲本明恢63(CK水稻)为研究材料,在模拟贫瘠营养条件下(不施肥、无靶标虫压)初步评估了转cry1 Ab/c抗虫基因水稻的生态适合度效应。结果表明:贫瘠条件下Bt水稻cry1 Ab/c基因的基本表达规律与大田条件下相似,但是Bt蛋白表达量更低。与亲本明恢63相比,Bt水稻在主要生长发育期的株高、叶绿素含量、叶形和根系等指标方面呈现出显著的生态适合度利益,Bt水稻实粒数、谷粒数、实粒重和千粒重这些重要繁殖指标也较亲本水稻显示出明显的生态适合度正效应,表明其种群在自然界中的生存能力和延续能力可能超越CK水稻,从而产生环境风险。

关键词: 转cry1 Ab/c基因水稻, 环境, 生态适合度, 风险评估

Abstract:

In a simulated nutrient-deficient soil (without fertilizer application or target insect stress),transgenic cry1 Ab /c rice HH1 (Bt rice) and its non-transgenic parental cultivar (CK rice) were planted for comparison of the two rice cultivars in ecological fitness. Results show that the Bt rice planted in either the simulated soil or the field followed a similar basic rule of cry1 Ab / c gene expression but the expression level of Bt protein was much lower in the former case. Bt rice showed significantly higher fitness benefits in plant height,chlorophyll content,leaf shape and root system development in most developmental stages than CK rice,and apparent positive effects of ecological fitness,as well,on reproductive parameters such as seed number per plant,seed number per spike,seed weight per plant and total weight per 1 000 seeds.All these findings suggest that Bt rice might exceed CK rice in survivability and reproductibility in nature,and hence it may pose some environmental risks.

Key words: cry1 Ab/c transgenic rice, environment, ecological fitness, risk assessment