生态与农村环境学报 ›› 2015, Vol. 31 ›› Issue (5): 760-766.doi: 10.11934/j.issn.1673-4831.2015.05.023

• 污染控制与修复 • 上一篇    下一篇

柠檬酸和EDTA对蓖麻生理特性和镉累积的影响

张玉芬,刘景辉,杨彦明,张雷   

  1. 内蒙古农业大学农学院
  • 收稿日期:2015-01-28 修回日期:2015-07-06 出版日期:2015-09-25 发布日期:2015-09-30
  • 通讯作者: 张玉芬 E-mail:zhyf103078@aliyun.com
  • 作者简介:张玉芬(1977-),女,内蒙古通辽人,副教授,博士,研究方向为土壤重金属污染的修复与治理。E-mail:zhyf103078@aliyun.com
  • 基金资助:

    农业部农业环境保护项目(20140420);国家自然科学基金(31360321);内蒙古自然科学基金(2015MS0340)

Effects of CA and EDTA on Physiological Characteristics of Ricinus communis L. and the Accumulation of Cd

ZHANG Yu-fen,LIU Jing-hui,YANG Yan-ming,ZHANG Lei   

  1. Agricultural college of Inner Mongolia Agricultural University
  • Received:2015-01-28 Revised:2015-07-06 Online:2015-09-25 Published:2015-09-30

摘要:

采用盆栽试验研究了螯合剂EDTA和柠檬酸(CA)单施或配施对蓖麻(Ricinus communis)生理特性及修复Cd污染土壤能力的影响。结果表明:单施EDTA显著降低了蓖麻地上生物量和叶绿素含量(P<0.05),显著影响了蓖麻叶片抗氧化酶活性(P<0.05),10 mmol·kg-1 EDTA甚至抑制了SOD和POD活性;CA与EDTA 联用可在一定程度上缓解EDTA对蓖麻的毒害作用, CA缓解了1和5 mmol·kg-1 EDTA导致的蓖麻地上生物量和叶绿素含量降低,缓解了1 mmol·kg-1 EDTA导致的MDA含量升高;单施5 mmol·kg-1 EDTA 蓖麻对Cd的富集和转移能力最高,但单株Cd富集量并不是最高的,而2 mmol·kg-1 CA与5 mmol·kg-1 EDTA配施却使蓖麻单株总Cd富集量和提取率达到最高,分别是对照的2.98和3.00倍。因此CA与EDTA联用在强化蓖麻修复Cd污染土壤中具有很好的应用潜力。

关键词: 蓖麻, CA, EDTA, 生物量,

Abstract:

A pot experiment was conducted to study effects of Citric acid (CA) and EDTA on physiological characteristics and Cd accumulation of R. communis. Results show that the application of EDTA alone significantly reduced the biomass of shoots and the content of chlorophyll of R. communis, evidently affected the activity of antioxidant enzyme in leaves of the plant(P<0.05), and even inhibited the activities of SOD and POD, when the application rate reached 10 mmol•kg-1. The application of EDTA and CA together reduced the toxicity of EDTA to R.communis to a certain extent, the effect of EDTA, at 1 or 5 mmol•kg-1, lowering the biomass of shoots and the content of chlorophyll of R.communis and the effect of EDTA, at 1 mmol•kg-1, increasing the content of MDA in the plant. The application of EDTA alone, at 5 mmol•kg-1, brought Cd accumulation and transfer capacity of the plant to the peak, two times as high as in CK. However, once 2 mmol•kg-1CA was applied in combination, the effect of EDTA reached a new peak, 3 times as high as in CK. Therefore, combined application of EDTA with Ca should be a practice of great potential in enhancing the effect of R.communis remedying Cd polluted soils.

Key words: Ricinus communis, CA, EDTA, biomass, Cd