生态与农村环境学报 ›› 2012, Vol. 28 ›› Issue (2): 213-216.doi:

• 污染控制与修复 • 上一篇    下一篇

一株脱硫菌的鉴定及其对H2S的降解能力

董晓莹, 赵勇, 寇巍, 李世密, 曲静霞, 张大雷   

  1. 辽宁省能源研究所
  • 收稿日期:2011-10-08 修回日期:2011-10-27 出版日期:2012-03-25 发布日期:2012-03-27
  • 通讯作者: 张大雷 辽宁省能源研究所 E-mail:daleizhang@163.com
  • 作者简介:董晓莹(1984-),女,辽宁营口市,助理研究员,硕士,主要研究方向为沼气发酵及脱硫。E-mail:dxy-1984@163.com
  • 基金资助:

    国家高技术研究发展计划(2010AA101604)

Identification of a Desulfurizing Strain and Its H2S Degrading Capacity

DONG  Xiao-Ying, ZHAO  Yong, KOU  Wei, LI  Shi-Mi, QU  Jing-Xia, ZHANG  Da-Lei   

  1. Liaoning Institute of Energy Resource
  • Received:2011-10-08 Revised:2011-10-27 Online:2012-03-25 Published:2012-03-27
  • Contact: ZHANG Da-Lei Liaoning Institute of Energy Resource E-mail:daleizhang@163.com

摘要: 取污水处理厂有生物活性的污泥,经富集培养、分离纯化得到1株脱硫活性较高的菌株CTD843-T-3,将其载入生物滴滤塔中对H2S进行连续脱硫反应。当最佳反应温度为30℃、pH为6.0、H2S进口浓度在500~3000 mg·m-3时,菌株对H2S的去除率可保持在92%以上,H2S最高负荷率可达6.5 g·m-3·h-1。通过形态生理特征和16S rDNA序列分析对CTD843-T-3进行鉴定,结果表明该菌株为椭球状,大小为0.4~0.6 μm,为革兰阴性菌,属假单胞菌属(Pseudomonas sp.)。

关键词: 硫化氢, 细菌, 16SrDNA, 假单胞菌属

Abstract: An active desulfurizing strain of bacteria CTD843-T-3 was isolated from bio-active sludge collected in a wastewater treatment plant and loaded into a bio-trickling reactor for consecutive desulfurization of H2S. When the reaction temperature was adjusted to 30℃, pH to 6.0,and the inlet concentration of H2S to 500~3000 mg·m-3,the H2S removal rate of the strain could be kept over 92%, and the maximum H2S loading rate could reach 6.5 g·m-3·h-1.Through the morphological, biochemical & physiological identification, and 16S rRNA sequence analysis, the strain was found to be ellipse-shaped, 0.4-0.6 μm in size,gram-negative,and a member of Pseudomonas.

Key words: hydrogen sulfide, bacterium, 16SrDNA, Pseudomonas

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