牧草在重金属污染土壤治理中的修复和综合利用潜力

doi:10.19741/j.issn.1673-4831.2020.0369

摘要/Abstract

摘要： 牧草易栽培，生物量大，产量高，在重金属污染土壤治理中具有良好的应用前景，既可作为农业生产的低积累饲用牧草，也可为重金属污染土壤修复提供高积累植物，是生物质能源植物和污染土壤修复的重要植物。综述了牧草在重金属污染土壤治理中的优势、积累特征、响应机制及主要牧草在重金属污染土壤治理中的应用和效益评估。分析了我国已发现的具有重金属污染土壤修复潜力的禾本科和豆科优良牧草类植物，对牧草吸收积累重金属的品种差异、单一/复合金属胁迫下牧草的响应及其机制等研究现状进行阐述，以期为利用牧草进行重金属污染土壤的治理和综合利用提供理论基础和实践参考。

关键词: 牧草, 重金属, 综合利用, 响应机制

Abstract: Forages are easy to cultivate and have high biomass and high yields, which endows its broad application prospect in heavy metal-contaminated soil. Forages are available as both low-accumulation for feed or high-accumulation for phytoremediation. Meanwhile, forages are also important plants for biomass energy and soil pollution remediation. The advantages, accumulation characteristics, response mechanism of forages in the treatment of heavy metal contaminated soil, and the application and benefit evaluation of main forage grasses in the treatment of heavy metal contaminated soil were reviewed. The excellent forage species of Gramineae and Leguminosae with potential for remediation of heavy metal contaminated soils in China were identified and analyzed. The varietal differences on absorbing and accumulating heavy metals, the response and mechanism of forage under single/compound metal stress were also described. The review is intented to provide theoretical basis and practical reference for the treatment of heavy metal contaminated soil by forages and the comprehensive utilization of the forages.

Key words: forage, heavy metal, comprehensive utilization, response mechanism

中图分类号:

姜娜, 杨京民, Gahonzire Bonheur, 李元, 祖艳群. 牧草在重金属污染土壤治理中的修复和综合利用潜力[J]. 生态与农村环境学报, 2021, 37(5): 545-554.

JIANG Na, YANG Jing-min, Gahonzire Bonheur, LI Yuan, ZU Yan-qun. Forage Grass for Phytoremediation of Heavy Metal Polluted Soils and the Potential for Its Comprehensive Utilization: A Review[J]. Journal of Ecology and Rural Environment, 2021, 37(5): 545-554.

参考文献

[1] 杨姝,李元,毕玉芬,等.紫花苜蓿对Cd胁迫的响应及品种差异研究进展[J].农业环境科学学报,2017,36(8):1453-1461.[YANG Shu,LI Yuan,BI Yu-fen,et al.Response and Intraspecific Differences of Alfalfa to Cadmium (Cd) Stress［J].Journal of Agro-environment Science,2017,36(8):1453-1461.］
[2] 王芹,何岸飞.植物吸收重金属动力学研究进展[J].绿色科技,2018(10):50-54.[WANG Qin,HE An-fei.Advances in Dynamics of Phytoremediation on Heavy-metal Contaminated Soils［J].Journal of Green Science and Technology,2018(10):50-54.］
[3] 朱阳春,张振华,钟小仙,等.牧草修复重金属污染土壤的研究进展[J].江苏农业科学,2018,46(4):1-6.
[4] 张芳,方溪,张丽静.草类对重金属胁迫的生理生化响应机制[J].草业科学,2012,29(4):534-541.[ZHANG Fang,FANG Xi,ZHANG Li-jing.Response of Physiology and Biochemistry of Grasses to Heavy Metal Stress［J].Pratacultural Science,2012,29(4):534-541.］
[5] AURANGZEB N,NISA S,BIBI Y,et al.Phytoremediation Potential of Aquatic Herbs from Steel Foundry Effluent[J].Brazilian Journal of Chemical Engineering,2014,31(4):881-886.
[6] PAZ-FERREIRO J,LU H,FU S,et al.Use of Phytoremediation and Biochar to Remediate Heavy Metal Polluted Soils:A Review[J].Solid Earth,2014,5(1):65-75.
[7] 张丽,赵颖,党晋华,等.牧草对重金属复合污染农田土壤的修复研究[C]//中国环境科学学会.2016中国环境科学学会学术年会论文集:3卷.北京:中国环境科学学会,2016:971-976.[ZHANG Li,ZHAO Ying,DANG Jin-hua,et al.Research on Forage Remediation of Heavy Metal Compound Contaminated Farmland Soil［C]//Chinese Society for Environmental Sciences.2016 Proceedings of the Annual Conference of the Chinese Society of Environmental Sciences:Vol. 3.Beijing:Chinese Society for Environmental Sciences,2016:971-976.］
[8] 王新,贾永锋.紫花苜蓿对土壤重金属富集及污染修复的潜力[J].土壤通报,2009,40(4):932-935.[WANG Xin,JIA Yong-feng.Heavy Metals Accumulation and Phytoremediation of Alfalfa in Contaminated Soil［J].Chinese Journal of Soil Science,2009,40(4):932-935.］
[9] 杨姝,贾乐,毕玉芬,等.7种紫花苜蓿对云南某铅锌矿区土壤镉铅的累积特征及品种差异[J].农业资源与环境学报,2018,35(3):222-228.[YANG Shu,JIA Le,BI Yu-fen,et al.Interspecific Differences and Accumulative Characteristics of Cadmium and Lead about Seven Alfalfa (Medicago sativa L.)Cultivars in a Lead-zinc Mine of Yunnan,China［J].Journal of Agricultural Resources and Environment,2018,35(3):222-228.］
[10] 陈惠君,谭玲,李取生,等.Cr/Pb低累积菜心品种筛选及其根际机理研究[J].农业环境科学学报,2016,35(7):1249-1256.[CHEN Hui-jun,TAN Ling,LI Qu-sheng,et al.Screening and Preliminary Rhizosphere Mechanisms of Low Cr/Pb Accumulation Cultivars of Chinese Flowering Cabbages (Brassica parachinensis L.)［J].Journal of Agro-environment Science,2016,35(7):1249-1256.］
[11] 陆阿飞.三江源区不同地区天然草地牧草营养成分分析[J].黑龙江畜牧兽医,2015(15):139-141.
[12] 黄德君,毛祝新,傅华.牧草中水溶性碳水化合物及其影响因素[J].草业学报,2011,20(6):270-278.[HUANG De-jun,MAO Zhu-xin,FU Hua.Characteristics of Herbage Water Soluble Carbohydrates and of Factors Influencing Them［J].Acta Prataculturae Sinica,2011,20(6):270-278.］
[13] DAI H P,WEI S H,POGRZEBA M,et al.Exogenous Jasmonic Acid Decreased Cu Accumulation by Alfalfa and Improved Its Photosynthetic Pigments and Antioxidant System[J].Ecotoxicology and Environmental Safety,2020,190:110176.
[14] DE ANICÉSIO É C A,MONTEIRO F A.Potassium Affects the Phytoextraction Potential of Tanzania Guinea Grass under Cadmium Stress[J].Environmental Science and Pollution Research,2019,26(29):30472-30484.
[15] 高秉婷,吴永贵,陈伟.Zn²⁺胁迫对4种草生长及抗逆生理特性的影响[J].江苏农业科学,2019,47(13):285-288.
[16] 张媛媛,李加米,李晓平,等.多年生黑麦草种子萌发及幼苗生长对镉胁迫的响应研究[J].乡村科技,2018(28):90-91.
[17] 李希铭,李金波,宋桂龙,等.35份草本植物镉吸收与富集特征比较[J].草业科学,2018,35(4):760-770.[LI Xi-ming,LI Jin-bo,SONG Gui-long,et al.Cadmium Tolerance and Absorption Characteristic of Herbaceous Plants［J].Pratacultural Science,2018,35(4):760-770.］
[18] 张龙冲,张秋玲,赵袁,等.4种铬污染地植物的富集特征及其种子萌发对铬(Cr⁶⁺)处理的响应[J].河南农业大学学报,2016,50(4):550-557.[ZHANG Long-chong,ZHANG Qiu-ling,ZHAO Yuan,et al.Enrichment Characteristics of Four Native Plant Species from Heavy Chromium Pollution Area and Their Response of Seed Germination to Chromium(Cr⁶⁺) Treatment［J].Journal of Henan Agricultural University,2016,50(4):550-557.］
[19] 孟楠,王萌,陈莉,等.不同草本植物间作对Cd污染土壤的修复效果[J].中国环境科学,2018,38(7):2618-2624.[MENG Nan,WANG Meng,CHEN Li,et al.Remediation Efficiency of Cd Polluted Soil by Intercropping with Herbaceous Plants ［J].China Environmental Science,2018,38(7):2618-2624.］
[20] 刘诗敏,高良敏,邱增羽,等.淮南部分采煤沉陷复垦区土地中植物重金属的富集特征比较研究[J].山东工业技术,2017(2):80-81.
[21] 龙健,黄昌勇,滕应,等.铜尾矿对五种牧草生长情况的影响[J].中国草地,2003,25(2):18-21.[LONG Jian,HUANG Chang-yong,TENG Ying,et al.Effects of Copper Mine Tailings on Growing Status of Five Forage Grass Species［J].Grassland of China,2003,25(2):18-21.］
[22] 郭丹,朱维琴,冯萍,等.不同品种牧草对高Cu、高Zn蚓粪中重金属的吸收和积累特性[J].杭州师范大学学报(自然科学版),2011,10(2):146-152.[GUO Dan,ZHU Wei-qin,FENG Ping,et al.Absorption and Accumulation of Heavy Metals by Different Forage from Vermicompost Enriched with High Cu and Zn［J].Journal of Hangzhou Normal University (Natural Science Edition),2011,10(2):146-152.］
[23] 孙金金,鱼小军,王金辉,等.重金属Cu²⁺、Cd²⁺和Pb²⁺对8种禾草种子萌发和幼苗生长的影响[J].草地学报,2018,26(3):673-683.[SUN Jin-jin,YU Xiao-jun,WANG Jin-hui,et al.Effects of Heavy Metals Cu²⁺,Cd²⁺ and Pb²⁺ on Seed Germination and Seedling Growth of 8 Grasses［J].Acta Agrestia Sinica,2018,26(3):673-683.］
[24] 覃建军,唐盛爽,蒋凯,等.象草在南方典型母质土壤中的镉修复效应[J].水土保持学报,2020,34(2):372-377.[QIN Jian-jun,TANG Sheng-shuang,JIANG Kai,et al.The Remediation Effect of Napier Grass to Cd-contaminated Soil with Typical Parent Materials in Southern China［J].Journal of Soil and Water Conservation,2020,34(2):372-377.］
[25] 徐磊,周静,梁家妮,等.巨菌草对Cu、Cd污染土壤的修复潜力[J].生态学报,2014,34(18):5342-5348.[XU Lei,ZHOU Jing,LIANG Jia-ni,et al.The Remediation Potential of Pennisetum sp. on Cu,Cd Contaminated Soil［J].Acta Ecologica Sinica,2014,34(18):5342-5348.］
[26] 谢华,赵雪梅,谢洲,等.皇竹草对酸与Cd污染农田土壤的治理效果及安全应用分析[J].农业环境科学学报,2016,35(3):478-484.[XIE Hua,ZHAO Xue-mei,XIE Zhou,et al.Phytoremediation Efficiency of Pennisetum hydridum for Acid- and Cadmium-polluted Soil and Its Safe Utilization［J].Journal of Agro-environment Science,2016,35(3):478-484.］
[27] 易自成,贺俊波,程华,等.镉对皇竹草构件生长及生理特性的影响[J].农业环境科学学报,2014,33(2):276-282.[YI Zi-cheng,HE Jun-bo,CHENG Hua,et al.Effects of Cd Polluted Soil on the Modular Growth and Physiological Characteristics of Pennisetum hydridum［J].Journal of Agro-environment Science,2014,33(2):276-282.］
[28] 李娟霞.紫云英对铜尾矿矿砂的耐性机理及修复潜力研究[D].南昌:江西财经大学,2015.[LI Juan-xia.The Tolerant Mechanisms and Potential for Cu Tailings Phytoremediation of Astragalus sinicus L.［D].Nanchang:Jiangxi University of Finance and Economics,2015.］
[29] 曾宪录,韩飞,钟艳梅,等.紫云英、田菁及光叶苕子富集硒、锶能力研究[J].福建农业科技,2017(1):15-19.[ZENG Xian-lu,HAN Fei,ZHONG Yan-mei,et al.Study on Selenium- and Strontium-concentrating Capacity of Milk Vetch,Sesbania and Smooth Vetch［J].Fujian Agricultural Science and Technology,2017(1):15-19.］
[30] 王博,田杰,龙林,等.重金属胁迫对白三叶种子萌发的影响[J].种子,2019,38(2):20-24.[WANG Bo,TIAN Jie,LONG Lin,et al.Effects of Heavy Metal Stress on Germination of Trifolium repens［J].Seed,2019,38(2):20-24.］
[31] 宋凤鸣,刘建华,吴彩琼,等.五种重金属胁迫对白三叶和红三叶种子萌发及幼苗生长的影响[J].亚热带植物科学,2014,43(4):276-280.[SONG Feng-ming,LIU Jian-hua,WU Cai-qiong,et al.Effects of Five Kinds of Heavy Metal Stress on Seed Germination and Seedling Growth of Trifolium repens and T. pratense［J].Subtropical Plant Science,2014,43(4):276-280.］
[32] 鱼小军,张建文,潘涛涛,等.铜、镉、铅对7种豆科牧草种子萌发和幼苗生长的影响[J].草地学报,2015,23(4):793-803.[YU Xiao-jun,ZHANG Jian-wen,PAN Tao-tao,et al.Effects of Heavy Metals:Copper,Cadmium and Lead on the Seed Germination and Seedling Growth of Leguminous Forage［J].Acta Agrestia Sinica,2015,23(4):793-803.］
[33] 李金波,李诗刚,宋桂龙,等.砷胁迫对黑麦草根系形态及养分吸收的影响[J].草业科学,2018,35(6):1385-1392.[LI Jin-bo,LI Shi-gang,SONG Gui-long,et al.Effect of Arsenic Stress on Root Morphological Parameters and Absorption of Nutrient Elements in Ryegrass［J].Pratacultural Science,2018,35(6):1385-1392.］
[34] DELHAIZE E,RYAN P R.Aluminum Toxicity and Tolerance in Plants[J].Plant Physiology,1995,107(2):315-321.
[35] FAROOQ M A,ALI S,HAMEED A,et al.Cadmium Stress in Cotton Seedlings:Physiological,Photosynthesis and Oxidative Damages Alleviated by Glycinebetaine[J].South African Journal of Botany,2016,104:61-68.
[36] SHEETAL K R,SINGH S D,ANAND A,et al.Heavy Metal Accumulation and Effects on Growth,Biomass and Physiological Processes in Mustard[J].Indian Journal of Plant Physiology,2016,21(2):219-223.
[37] 张春雨,王海娟,王宏镔.赤霉素介导下植物对重金属的耐性机理[J].生态与农村环境学报,2020,36(2):137-144.[ZHANG Chun-yu,WANG Hai-juan,WANG Hong-bin.Mechanisms of Plant Tolerance to Heavy Metals Mediated by Gibberellic Acid［J].Journal of Ecology and Rural Environment,2020,36(2):137-144.］
[38] PARSAMANESH S,SADEGHI H.The Phytoremediation Effect of Medicago scutellata (L.) Mill. on Soils under Cd-water Stress:A Good Choice for Contaminated Dry Lands[J].Environmental Science and Pollution Research,2019,26(28):29065-29073.
[39] 张茂,徐彦红,席溢,等.铅、锌、镉胁迫对多年生黑麦草生长及生理生化特性的影响[J].中国农业科技导报,2021,23(3):41-50.[ZHANG Mao,XU Yan-hong,XI Yi,et al.［Effects of Single Stress of Pb²⁺,Zn²⁺ and Cd²⁺ on Growth,Physiological and Biochemical Characteristics of Perennial Ryegrass［J].Journal of Agricultural Science and Technology,2021,23(3):41-50.］
[40] ZHONG W X,XIE C C,HU D,et al.Effect of 24-epibrassinolide on Reactive Oxygen Species and Antioxidative Defense Systems in Tall Fescue Plants under Lead Stress[J].Ecotoxicology and Environmental Safety,2020,187:109831-109841.
[41] LU Y,LI X R,HE M Z,et al.Seedlings Growth and Antioxidative Enzymes Activities in Leaves under Heavy Metal Stress Differ between Two Desert Plants:A Perennial (Peganum harmala) and an Annual (Halogeton glomeratus) Grass[J].Acta Physiologiae Plantarum,2010,32(3):583-590.
[42] FLORES-CÁCERES M L,HATTAB S,HATTAB S,et al.Specific Mechanisms of Tolerance to Copper and Cadmium Are Compromised by a Limited Concentration of Glutathione in Alfalfa Plants[J].Plant Science,2015,233:165-173.
[43] ASLAM S,SHARIF F,KHAN A U.Effect of Lead and Cadmium on Growth of Medicago sativa L. and Their Transfer to Food Chain[J].Journal of Animal and Plant Sciences,2015,25(2):472-477.
[44] 白哲,董馨岚,李铭红,等.铜胁迫对4种牧草种子萌发及幼苗生长的影响[C]//2019中国环境科学学会科学技术年会论文集.西安:[出版者不详],2019:654-659.
[45] 韩晓姝,曹成有,姚金冬,等.铜、镉对三种豆科植物生长及氮磷钾含量的影响[J].生态学杂志,2009,28(11):2250-2256.[HAN Xiao-shu,CAO Cheng-you,YAO Jin-dong,et al.Effects of Copper and Cadmium on Leguminous Plant Growth and Nutrient Uptake［J].Chinese Journal of Ecology,2009,28(11):2250-2256.］
[46] 贾乐,杨姝,祖艳群,等.Cd胁迫对不同品种紫花苜蓿生长及Cd累积的影响[J].安徽农业科学,2018,46(22):61-64.[JIA Le,YANG Shu,ZU Yan-qun,et al.Effect of Cd Stress on Growth and Cd Accumulation of Different Alfalfa Varieties［J].Journal of Anhui Agricultural Sciences,2018,46(22):61-64.］
[47] 侯新村,范希峰,武菊英,等.草本能源植物修复重金属污染土壤的潜力[J].中国草地学报,2012,34(1):59-64,76.[HOU Xin-cun,FAN Xi-feng,WU Ju-ying,et al.Potentiality of Herbaceous Bioenergy Plants in Remediation of Soil Contaminated by Heavy Metals［J].Chinese Journal of Grassland,2012,34(1):59-64,76.］
[48] MEHARG A A,MACNAIR M R.Suppression of the High Affinity Phosphate Uptake System:A Mechanism of Arsenate Tolerance in Holcus lanatus L.[J].Journal of Experimental Botany,1992,43(4):519-524.
[49] MURTAZA G,JAVED W,HUSSAIN A,et al.Soil-applied Zinc and Copper Suppress Cadmium Uptake and Improve the Performance of Cereals and Legumes[J].International Journal of Phytoremediation,2017,19(2):199-206.
[50] 周启星.镉-砷污染苜蓿的联合效应及机理的研究[J].应用基础与工程科学学报,1994,2(1):81-87.[ZHOU Qi-xing.Joint Effects and Mechanisms of Cd and As Simultaneously Polluting Alfalfa［J].Journal of Basic Science and Engineering,1994,2(1):81-87.］
[51] POBLACIONES M J,DAMON P,RENGEL Z.Foliar Zinc Biofortification Effects in Lolium rigidum and Trifolium subterraneum Grown in Cadmium-contaminated Soil[J].PLoS One,2017,12(9):e0185395.
[52] LIU H W,WANG H,GAO W F,et al.Phytoremediation of Three Herbaceous Plants to Remove Metals from Urban Runoff[J].Bulletin of Environmental Contamination and Toxicology,2019,103:336-341.
[53] ONDER S,DURSUN S,GEZGIN S,et al.Determination of Heavy Metal Pollution in Grass and Soil of City Centre Green Areas (Konya,Turkey) [J].Polish Journal of Environmental Studies,2007,16(1):145-154.
[54] KABAIJA E,SMITH O B.Effect of Season,Fertilizer Application and Age of Regrowth on Mineral Content of Guinea Grass (Panicum maximum Schum) and Giant Star Grass (Cynodon nlemfuensis Chedda):FAO Corporate Document [R].Addis Ababa,Ethiopia:Respository International Livestock Centre for Africa,1988:440-448.
[55] MATTHEWS-AMUNE O C,KAKULU S.Determination of Heavy Metals in Forage Grasses [Carpet Grass (Axonopus ompressus),Guinea Grass (Panicum maximum) and Elephant Grass (Pennisetum purpureum)]in the Vicinity of Itakpe Iron Ore Mine,Nigeria[J].International Journal of Pure and Applied Sciences and Technology,2012,13(2):16-25.
[56] YAMAJI N,MA J F.The Node,a Hub for Mineral Nutrient Distribution in Graminaceous Plants[J].Trends in Plant Science,2014,19(9):556-563.
[57] 王新海,毕玉芬,奎嘉祥.耐酸性紫花苜蓿的研究进展[J].云南农业大学学报,2005,20(5):705-709.[WANG Xin-hai,BI Yu-fen,KUI Jia-xiang.Progress of the Research on Alfalfa with Acidity Tolerance［J].Journal of Yunnan Agricultural University,2005,20(5):705-709.］
[58] ZHANG J,QIAN Y G,CHEN Z B,et al.Lead-induced Oxidative Stress Triggers Root Cell Wall Remodeling and Increases Lead Absorption through Esterification of Cell Wall Polysaccharide[J].Journal of Hazardous Materials,2020,385:121524-121536.
[59] LI X X,CUI X W,ZHANG X,et al.Combined Toxicity and Detoxification of Lead,Cadmium and Arsenic in Solanum nigrum L[J].Journal of Hazardous Materials,2020,389:121874.
[60] 刘俊祥,许新桥,钱永强,等.多年生黑麦草抗氧化酶和植物络合素对Cd²⁺胁迫的应答[J].生态学杂志,2013,32(7):1787-1793.[LIU Jun-xiang,XU Xin-qiao,QIAN Yong-qiang,et al.Responses of Antioxidative Enzymes and Phytochelatins in Lolium perenne to Cd²⁺ Stress［J].Chinese Journal of Ecology,2013,32(7):1787-1793.］
[61] 王慧忠,李鹃.重金属镉、铅对多年生黑麦草细胞内几种抗氧化酶基因表达的影响[J].农业环境科学学报,2008,27(6):2371-2376.[WANG Hui-zhong,LI Juan.Effect of Cadmium Ion and Lead Ion Induced Stress on Anti-oxidative Genes Expression in Perennial Ryegrass Cells［J].Journal of Agro-environment Science,2008,27(6):2371-2376.］
[62] GONG Z Z,XIONG L M,SHI H Z,et al.Plant Abiotic Stress Response and Nutrient Use Efficiency[J].Science China:Life Sciences,2020,63(5):635-674.
[63] LI J C,GUO J B,XU W Z,et al.Enhanced Cadmium Accumulation in Transgenic Tobacco Expressing the Phytochelatin Synthase Gene of Cynodon Dactylon L[J].Journal of Integrative Plant Biology,2006,48(8):928-937.
[64] 徐畅,何好,李国良,等.紫花苜蓿1型金属硫蛋白基因的表达及抗逆性分析[J].草业学报,2018,27(4):89-97.[XU Chang,HE Hao,LI Guo-liang,et al.Expression of Type 1 Metallothionein Gene from Medicago sativa and Analysis of Its Function in Stress Tolerance［J].Acta Prataculturae Sinica,2018,27(4):89-97.］
[65] KIEFFER P,DOMMES J,HOFFMANN L,et al.Quantitative Changes in Protein Expression of Cadmium-exposed Poplar Plants[J].Proteomics,2008,8(12):2514-2530.
[66] 宋文静,王凭青,张宝云,等.紫花苜蓿铝激活半型ABC转运子基因MsSTAR2的耐铝性分析[J].农业生物技术学报,2013,21(5):522-529.[SONG Wen-jing,WANG Ping-qing,ZHANG Bao-yun,et al.MsSTAR2 Encodes a Half-type ATP-binding Cassette Transporter Protein That Is Induced by Aluminum in Medicago sativa L.［J].Journal of Agricultural Biotechnology,2013,21(5):522-529.］
[67] 赵彩春,李一星,陈大松,等.紫云英2个转脂蛋白基因在共生及重金属镉胁迫条件下的表达特征[J].华中农业大学学报,2011,30(1):18-22.[ZHAO Cai-chun,LI Yi-xing,CHEN Da-song,et al.Expression Patterns of Two Lipid Transfer Protein Encoding Genes of Astragalus sinicus under Symbiosis and Heavy Metal Cadmium Stress Conditions［J].Journal of Huazhong Agricultural University,2011,30(1):18-22.］
[68] LEE K W,CHOI G J,KIM K Y,et al.Transgenic Expression of MsHsp23 Confers Enhanced Tolerance to Abiotic Stresses in Tall Fescue[J].Asian-Australasian Journal of Animal Sciences,2012,25(6):818-823.
[69] 伍佰鑫,孙鏖,易康乐.牧草的研究进展[J].中国畜牧业,2019(7):57-60.[WU Bai-xin,SUN Ao,YI Kang-Le.Research Progress and Targeting of Pasture［J].China Animal Industry,2019(7):57-60.］
[70] 张杏锋,夏汉平,李志安,等.牧草对重金属污染土壤的植物修复综述[J].生态学杂志,2009,28(8):1640-1646.[ZHANG Xing-feng,XIA Han-ping,LI Zhi-an,et al.Forage Grass in Phytoremediation of Heavy Metals-contaminated Soils:A Review［J].Chinese Journal of Ecology,2009,28(8):1640-1646.］
[71] TENG Y,LUO Y M,HUANG C Y,et al.Tolerance of Grasses to Heavy Metals and Microbial Functional Diversity in Soils Contaminated with Copper Mine Tailings[J].Pedosphere,2008,18(3):363-370.
[72] ZHAO A Q,GAO L Y,CHEN B Q,et al.Phytoremediation Potential of Miscanthus sinensis for Mercury-polluted Sites and Its Impacts on Soil Microbial Community[J].Environmental Science and Pollution Research,2019,26(34):34818-34829.
[73] RUSINOWSKI S,KRZYAK J,CLIFTON-BROWN J,et al.New Miscanthus Hybrids Cultivated at a Polish Metal-contaminated Site Demonstrate High Stomatal Regulation and Reduced Shoot Pb and Cd Concentrations[J].Environmental Pollution,2019,252:1377-1387.
[74] ZHANG X F,XIA H P,LI Z A,et al.Potential of Four Forage Grasses in Remediation of Cd and Zn Contaminated Soils[J].Bioresource Technology,2010,101(6):2063-2066.
[75] GHOLINEJAD B,KHASHIJ S,GHORBANI F,et al.Effects of Lead Ions on Germination,Initial Growth,and Physiological Characteristics of Lolium perenne L. Species and Its Bioaccumulation Potential[J].Environmental Science and Pollution Research International,2020,27(10):11155-11163.
[76] LI N Y,GUO B,LI H,et al.Effects of Double Harvesting on Heavy Metal Uptake by Six Forage Species and the Potential for Phytoextraction in Field[J].Pedosphere,2016,26(5):717-724.
[77] LIU M H,ZHAO Z J,CHEN L,et al.Influences of Arbuscular Mycorrhizae,Phosphorus Fertiliser and Biochar on Alfalfa Growth,Nutrient Status and Cadmium Uptake[J].Ecotoxicology and Environmental Safety,2020,196:110537.
[78] XIAO Y,LIU M H,CHEN L,et al.Growth and Elemental Uptake of Trifolium repens in Response to Biochar Addition,Arbuscular Mycorrhizal Fungi and Phosphorus Fertilizer Applications in Low-Cd-polluted Soils[J].Environmental Pollution,2020,260:113761.
[79] JU W L,LIU L,JIN X L,et al.Co-inoculation Effect of Plant-growth-promoting Rhizobacteria and Rhizobium on EDDS Assisted Phytoremediation of Cu Contaminated Soils[J].Chemosphere,2020,254:126724.
[80] WU J Z,KAMAL N,HAO H H,et al.Endophytic Bacillus megaterium BM18-2 Mutated for Cadmium Accumulation and Improving Plant Growth in Hybrid Pennisetum[J].Biotechnology Reports,2019,24:e00374.