微生物燃料电池(MFC)型生物毒性传感器用于重金属离子检测的研究进展

doi:10.19741/j.issn.1673-4831.2019.0957

摘要/Abstract

摘要： 微生物燃料电池（microbial fuel cell，MFC）型生物毒性传感器以其低成本、实时快速、操作简单和能实现污染物在线自动检测等特点在环境监测方面有着广泛的应用前景。总结了近十年应用MFC型生物毒性传感器对重金属离子进行检测与预警的研究情况。从MFC型生物毒性传感器的原理出发，介绍了评价MFC型生物毒性传感器性能的主要指标。从MFC的构型、膜类型、电极材料、控制模式和接种微生物5个方面分析了其对重金属检测性能的影响。展望了未来MFC型生物毒性传感器可能的研究方向：（1）筛选出具有特异性、灵敏度高和恢复速度快的阳极产电微生物菌种，实现对某一特定重金属离子的检测；（2）结合新型材料工艺实现传感器的微型化和集成化；（3）结合5G技术，实现MFC型生物毒性传感器的远程在线实时监测以及智能化拓展。

关键词: 微生物燃料电池, 生物传感器, 重金属, 电化学, 环境监测

Abstract: Microbial fuel cell (MFC) based toxicity biosensors have shown wide application prospects in environmental monitoring due to the superiority of low cost, real-time, simple operation and online automatic detection. It was summarized that the research status of MFC-based toxicity biosensors for detecting and warning of heavy metal ions in recent ten years. Main indicators of the functions of biosensors were introduced based on the principle of the MFC-based toxicity biosensors. The performance of the MFC detecting heavy metals was analyzed from five aspects including its configuration, membrane type, electrode material, control mode and inoculated microorganism. Furthermore, the following potential future research directions of MFC-based toxicity biosensors were prospected:(1) To screen the electricigens with good specificity, high sensitivity and fast recovery for the detection of a specific heavy metal ion; (2) To achieve the miniaturization and integration of biosensors based on the new material technology; (3) To realize the remote online real-time monitoring and intelligent of MFC-based toxicity biosensors by the combination with 5th-generation technology.

Key words: microbial fuel cell, biosensor, heavy metal, electrochemistry, environmental monitoring

中图分类号:

X382

鲁汭, 林莉莉, 肖恩荣, 吴振斌. 微生物燃料电池(MFC)型生物毒性传感器用于重金属离子检测的研究进展[J]. 生态与农村环境学报, 2021, 37(1): 1-9.

LU Rui, LIN Li-li, XIAO En-rong, WU Zhen-bin. Review of Development in Heavy Metals Monitoring with Microbial Fuel Cell-based Toxicity Biosensor[J]. Journal of Ecology and Rural Environment, 2021, 37(1): 1-9.

参考文献

[1] EMENIKE P C,NERIS J B,TENEBE I T,et al.Estimation of Some Trace Metal Pollutants in River Atuwara Southwestern Nigeria and Spatio-temporal Human Health Risks Assessment[J].Chemosphere,2020,239:124770.
[2] 张又文,韩建华,涂棋,等.天津市郊农田土壤重金属积累特征及评价[J].生态与农村环境学报,2019,35(11):1445-1452.[ZHANG You-wen,HAN Jian-hua,TU Qi,et al.Accumulation Characteristics and Evaluation of Heavy Metals in Suburban Farmland Soils of Tianjin[J].Journal of Ecology and Rural Environment,2019,35(11):1445-1452.]
[3] 梁西振.重金属检测技术的应用及发展探讨[J].世界有色金属,2018(17):276-276,278.[LIANG Xi-zhen.Discussion on the Application and Development of Heavy Metal Detection Technology[J].World Nonferrous Metals,2018(17):276-276,278.]
[4] MALIK L A,BASHIR A,QUREASHI A,et al.Detection and Removal of Heavy Metal Ions:A Review[J].Environmental Chemistry Letters,2019,17(4):1495-1521.
[5] 徐建英,赵春桃,魏东斌.生物毒性检测在水质安全评价中的应用[J].环境科学,2014,35(10):3991-3997.[XU Jian-ying,ZHAO Chun-tao,WEI Dong-bin.Toxicity Tests and Their Application in Safety Assessment of Water Quality[J].Environmental Science,2014,35(10):3991-3997.]
[6] CHOULER J,DI LORENZO M.Water Quality Monitoring in Developing Countries:Can Microbial Fuel Cells Be the Answer?[J].Biosensors,2015,5(3):450-470.
[7] JIA H,YANG G,WANG J,et al.Performance of a Microbial Fuel Cell-based Biosensor for Online Monitoring in an Integrated System Combining Microbial Fuel Cell and Upflow Anaerobic Sludge Bed Reactor[J].Bioresource Technology,2016,218:286-293.
[8] SONG N,YAN Z S,XU H C,et al.Development of a Sediment Microbial Fuel Cell-based Biosensor for Simultaneous Online Monitoring of Dissolved Oxygen Concentrations along Various Depths in Lake Water[J].Science of the Total Environment,2019,673:272-280.
[9] YAMASHITA T,OOKAWA N,ISHIDA M,et al.A Novel Open-type Biosensor for the In-situ Monitoring of Biochemical Oxygen Demand in an Aerobic Environment[J].Scientific Reports,2016,6:38552.
[10] DI LORENZO M,THOMSON A R,SCHNEIDER K,et al.A Small-scale Air-cathode Microbial Fuel Cell for On-line Monitoring of Water Quality[J].Biosensors and Bioelectronics,2014,62:182-188.
[11] LOGAN B E,HAMELERS B,ROZENDAL R,et al.Microbial Fuel Cells:Methodology and Technology[J].Environmental Science&Technology,2006,40(17):5181-5192.
[12] LOGAN B E.Exoelectrogenic Bacteria that Power Microbial Fuel Cells[J].Nature Reviews Microbiology,2009,7(5):375-381.
[13] SU L,JIA W Z,HOU C J,et al.Microbial Biosensors:A Review[J].Biosensors&Bioelectronics,2011,26(5):1788-1799.
[14] JIANG Y,YANG X F,LIANG P,et al.Microbial Fuel Cell Sensors for Water Quality Early Warning Systems:Fundamentals,Signal Resolution,Optimization and Future Challenges[J].Renewable and Sustainable Energy Reviews,2018,81:292-305.
[15] CHANG I S,JANG J K,GIL G C,et al.Continuous Determination of Biochemical Oxygen Demand Using Microbial Fuel Cell Type Biosensor[J].Biosensors&Bioelectronics,2004,19(6):607-613.
[16] MODIN O,WILÉN B M.A Novel Bioelectrochemical BOD Sensor Operating with Voltage Input[J].Water Research,2012,46(18):6113-6120.
[17] WU L C,WANG G H,TSAI T H,et al.Three-stage Single-chambered Microbial Fuel Cell Biosensor Inoculated with Exiguobacterium aestuarii YC₂11 for Continuous Chromium (Ⅵ) Measurement[J].Sensors,2019,19(6):1418.
[18] ZHAO T,XIE B Z,YI Y,et al.Sequential Flowing Membrane-less Microbial Fuel Cell Using Bioanode and Biocathode as Sensing Elements for Toxicity Monitoring[J].Bioresource Technology,2019,276:276-280.
[19] KIM M,SIK HYUN M,GADD G M,et al.A Novel Biomonitoring System Using Microbial Fuel Cells[J].Journal of Environmental Monitoring,2007,9(12):1323-1328.
[20] 乔军晶.基于微生物燃料电池的重金属浓度检测技术的电化学参数选择与优化[D].上海:华东理工大学,2016.[QIAO Jun-jing.Electrochemical Parameter Selection and Optimization of Heavy Metal Detection Technology Based on Microbial Fuel Cell[D].Shanghai:East China University of Science and Technology,2016.]
[21] STEIN N E,HAMELERS H V M,BUISMAN C N J.Influence of Membrane Type,Current and Potential on the Response to Chemical Toxicants of a Microbial Fuel Cell Based Biosensor[J].Sensors and Actuators B:Chemical,2012,163(1):1-7.
[22] 高艳梅,海热提,王晓慧,等.双室微生物燃料电池重金属毒性传感器的研制[J].环境工程学报,2017,11(10):5400-5408.[GAO Yan-mei,HAI Re-ti,WANG Xiao-hui,et al.Development of Heavy Metals Toxic Sensors for Double-microbial Fuel Cell[J].Chinese Journal of Environmental Engineering,2017,11(10):5400-5408.]
[23] DENG H,JIANG Y B,ZHOU Y W,et al.Using Electrical Signals of Microbial Fuel Cells to Detect Copper Stress on Soil Microorganisms[J].European Journal of Soil Science,2015,66(2):369-377.
[24] SHEN Y J,WANG M,CHANG I S,et al.Effect of Shear Rate on the Response of Microbial Fuel Cell Toxicity Sensor to Cu (Ⅱ)[J].Bioresource Technology,2013,136:707-710.
[25] YI Y,LI X Y,JIANG X H,et al.Assessing the Solitary and Joint Biotoxicities of Heavy Metals and Acephate Using Microbial Fuel Cell[C]//Proceedings of the International Conference on Advances in Energy,Environment and Chemical Engineering,September 26-27,2015.Changsha,China;Paris,France:Atlantis Press,2015:694-699.
[26] YU D B,BAI L,ZHAI J F,et al.Toxicity Detection in Water Containing Heavy Metal Ions with a Self-powered Microbial Fuel Cell-based Biosensor[J].Talanta,2017,168:210-216.
[27] PATIL S,HARNISCH F,SCHRÖDER U.Toxicity Response of Electroactive Microbial Biofilms:A Decisive Feature for Potential Biosensor and Power Source Applications[J].Chemphyschem,2010,11(13):2834-2837.
[28] STEIN N E,HAMELERS H V M,BUISMAN C N J.Stabilizing the Baseline Current of a Microbial Fuel Cell-based Biosensor through Overpotential Control under Non-toxic Conditions[J].Bioelectrochemistry (Amsterdam,Netherlands),2010,78(1):87-91.
[29] JIANG Y,LIANG P,ZHANG C Y,et al.Enhancing the Response of Microbial Fuel Cell Based Toxicity Sensors to Cu (Ⅱ) with the Applying of Flow-through Electrodes and Controlled Anode Potentials[J].Bioresource Technology,2015,190:367-372.
[30] 常定明.重金属离子在微生物燃料电池阳极中的行为[D].上海:华东理工大学,2015.[CHANG Ding-ming.The Behavior of Heavy Metal Ions in Microbial Fuel Cell[D].Shanghai:East China University of Science and Technology,2015.]
[31] WANG G H,CHENG C Y,LIU M H,et al.Utility of Ochrobactrum Anthropi YC152 in a Microbial Fuel Cell as an Early Warning Device for Hexavalent Chromium Determination[J].Sensors,2016,16(8):1272.
[32] XU Z H,LIU B C,DONG Q C,et al.Flat Microliter Membrane-based Microbial Fuel Cell as "On-line Sticker Sensor" for Self-supported in Situ Monitoring of Wastewater Shocks[J].Bioresource Technology,2015,197:244-251.
[33] STEIN N E,HAMELERS H V M,BUISMAN C N J.The Effect of Different Control Mechanisms on the Sensitivity and Recovery Time of a Microbial Fuel Cell Based Biosensor[J].Sensors and Actuators,B:Chemical,2012,171/172:816-821.
[34] 吴锋,刘志,周奔,等.单室MFC型生物毒性传感器对重金属离子的检测研究[J].环境科学,2010,31(7):1596-1600.[WU Feng,LIU Zhi,ZHOU Ben,et al.Development of a Low-cost Single Chamber Microbial Fuel Cell Type BOD Sensor[J].Chinese Journal of Environmental Science,2010,31(7):1596-1600.]
[35] 吴永贵,黄建国,袁玲.利用隆线溞趋光行为评价铬的生物毒性[J].应用生态学报,2005,16(1):171-174.[WU Yong-gui,HUANG Jian-guo,YUAN Ling.Phototactic Behavior of Daphnia carinata as an Indicator of Chromium Biotoxicity[J].Chinese Journal of Applied Ecology,2005,16(1):171-174.]
[36] LIU H,LOGAN B E.Electricity Generation Using an Air-cathode Single Chamber Microbial Fuel Cell in the Presence and Absence of a Proton Exchange Membrane[J].Environmental Science&Technology,2004,38(14):4040-4046.
[37] FRAIWAN A,MUKHERJEE S,SUNDERMIER S,et al.A Paper-based Microbial Fuel Cell:Instant Battery for Disposable Diagnostic Devices[J].Biosensors and Bioelectronics,2013,49:410-414.
[38] PARK D H,ZEIKUS J G.Improved Fuel Cell and Electrode Designs for Producing Electricity from Microbial Degradation[J].Biotechnology and Bioengineering,2003,81(3):348-355.
[39] LI Y,WU Y N,PURANIK S,et al.Metals as Electron Acceptors in Single-chamber Microbial Fuel Cells[J].Journal of Power Sources,2014,269:430-439.
[40] CHENG S A,LIU H,LOGAN B E.Power Densities Using Different Cathode Catalysts (Pt and CoTMPP) and Polymer Binders (Nafion and PTFE) in Single Chamber Microbial Fuel Cells[J].Environmental Science&Technology,2006,40(1):364-369.
[41] ZHAO F,HARNISCH F,SCHRÖDER U,et al.Application of Pyrolysed Iron (Ⅱ) Phthalocyanine and CoTMPP Based Oxygen Reduction Catalysts as Cathode Materials in Microbial Fuel Cells[J].Electrochemistry Communications,2005,7(12):1405-1410.
[42] HE Z,ANGENENT L.Application of Bacterial Biocathodes in Microbial Fuel Cells[J].Electroanalysis,2006,18(19/20):2009-2015.
[43] JIN X D,LI X H,ZHAO N N,et al.Bio-electrolytic Sensor for Rapid Monitoring of Volatile Fatty Acids in Anaerobic Digestion Process[J].Water Research,2017,111:74-80.
[44] LOVLEY D R.Bug Juice:Harvesting Electricity with Microorganisms[J].Nature Reviews:Microbiology,2006,4(7):497-508.
[45] 党政,代群威,赵玉连,等.生物矿化在重金属污染治理领域的研究进展[J].环境科学研究,2018,31(7):1182-1192.[DANG Zheng,DAI Qun-wei,ZHAO Yu-lian,et al.Research Progress of Biomineralization in the Treatment of Heavy Metal Contamination[J].Research of Environmental Sciences,2018,31(7):1182-1192.]
[46] RABAEY K,BOON N,SICILIANO S D,et al.Biofuel Cells Select for Microbial Consortia that Self-mediate Electron Transfer[J].Applied and Environmental Microbiology,2004,70(9):5373-5382.
[47] 季斌,陈威,樊杰,等.产脲酶微生物诱导钙沉淀及其工程应用研究进展[J].南京大学学报(自然科学),2017(1):191-198.[JI Bin,CHEN Wei,FAN Jie,et al.Research Progress on Microbial Induced Calcium Precipitation by Ureolytic Microbes and Its Prospect in Engineering Application[J].Journal of Nanjing University (Natural Sciences),2017(1):191-198.]
[48] BEAZLEY M J,MARTINEZ R J,SOBECKY P A,et al.Nonreductive Biomineralization of Uranium (Ⅵ) Phosphate via Microbial Phosphatase Activity in Anaerobic Conditions[J].Geomicrobiology Journal,2009,26(7):431-441.
[49] LIU L H,LEE D J,WANG A J,et al.Isolation of Fe (Ⅲ)-reducing Bacterium,Citrobacter sp.LAR-1,for Startup of Microbial Fuel Cell[J].International Journal of Hydrogen Energy,2016,41(7):4498-4503.
[50] 李明,梁湘,骆健美,等.一株产电菌嗜根考克氏菌(Kocuria rhizophila)的分离及其产电性能优化[J].环境科学学报,2015,35(10):3078-3087.[LI Ming,LIANG Xiang,LUO Jian-mei,et al.Isolation of an Exoelectrogen Kocuria rhizophila from the Anodic Biofilm and Optimization of Its Power Generation[J].Acta Scientiae Circumstantiae,2015,35(10):3078-3087.]
[51] LEE Y Y,KIM T G,CHO K S.Enhancement of Electricity Production in a Mediatorless Air-cathode Microbial Fuel Cell Using Klebsiella sp. IR21[J].Bioprocess and Biosystems Engineering,2016,39(6):1005-1014.
[52] PHAM C A,JUNG S J,PHUNG N T,et al.A Novel Electrochemically Active and Fe (Ⅲ)-reducing Bacterium Phylogenetically Related to Aeromonas hydrophila,Isolated from a Microbial Fuel Cell[J].FEMS Microbiology Letters,2003,223(1):129-134.
[53] 林稚兰,田哲贤.微生物对重金属的抗性及解毒机理[J].微生物学通报,1998,25(1):36-39.